Fig 1: Mindin-mediated signalling pathway analysis. A, Western blot analysis using antibodies against ERK1/2, phospho-ERK1/2, p65-NF-?B and phospho-p65-NF-?B and protein lysates from mindin, PCMV4, shMindin and PU6 cells. GAPDH was used as a loading control. B, Western blot analysis of the phosphorylation level of ERK1/2 in mindin-overexpressing (upper panel) or knock-down (lower panel) tumour tissues from tumour subcutaneous implantation model mice. Tubulin was used as a protein loading control (n = 5). C and D, Western blot analysis of c-Fos, FosB, c-Jun, FRA1, CDK4, CDK6, CyclinD1, CyclinD3, P15 and P27 expression in the stable cell lines and their controls